ARG54053
anti-Cyclin D2 antibody
anti-Cyclin D2 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Cyclin D2 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | Cyclin D2 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Cyclin D2 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | KIAK0002; MPPH3; G1/S-specific cyclin-D2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1.9 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCND2 |
| 全名 | cyclin D2 |
| 背景介绍 | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase.Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.Also substrate for SMAD3,phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity.Component of the ternary complex,cyclin D2/CDK4/CDKN1B,required for nuclear translocation and activity of the cyclin D-CDK4 complex by similarity. |
| 生物功能 | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Membrane. Note: Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation. |
检测图片 (1) Click the Picture to Zoom In
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ARG54053 anti-Cyclin D2 antibody WB image
Western blot: CHO-K1 transfected by Cyclin D2-fragment EGFP and stained with ARG54053 anti-Cyclin D2 antibody at 1:1000 dilution.
