ARG51827
anti-Cyclin B1 phospho (Ser147) antibody
anti-Cyclin B1 phospho (Ser147) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cyclin B1 phospho (Ser147) |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cyclin B1 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1. |
| 偶联标记 | Un-conjugated |
| 別名 | G2/mitotic-specific cyclin-B1; CCNB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCNB1 |
| 全名 | cyclin B1 |
| 背景介绍 | The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| 生物功能 | Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
检测图片 (3) Click the Picture to Zoom In
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ARG51827 anti-Cyclin B1 phospho (Ser147) antibody WB image
Western blot: Extracts from MDA cells untreated or treated with Anisomycin stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody.
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ARG51827 anti-Cyclin B1 phospho (Ser147) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51827 anti-Cyclin B1 phospho (Ser147) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51827 anti-Cyclin B1 phospho (Ser147) antibody.
