ARG62456

anti-Cyclin B1 antibody [V152]

anti-Cyclin B1 antibody [V152] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody

概述

产品描述 Mouse Monoclonal antibody [V152] recognizes Cyclin B1
反应物种 Hu
预测物种 Ms
应用 FACS, ICC/IF, IHC-Fr, IHC-P, WB
特异性 Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression.
宿主 Mouse
克隆 Monoclonal
克隆号 V152
同位型 IgG1
靶点名称 Cyclin B1
抗原物种 Hamster
抗原 His-tagged Hamster Cyclin B1 expressed in bacteria
偶联标记 Un-conjugated
別名 G2/mitotic-specific cyclin-B1; CCNB

应用说明

应用建议
应用 推荐稀释比
FACSAssay dependent
ICC/IFAssay dependent
IHC-Fr1 - 3 µg/ml
IHC-P1 - 3 µg/ml
WB1 - 3 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Protein G purified
缓冲液 PBS (pH 7.4) and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 891 Human CCNB1

Swiss-port # P14635 Human G2/mitotic-specific cyclin-B1

基因名称 Ccnb1
全名 cyclin B1
背景介绍 The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
产品亮点 Related Antibody Duos and Panels:
ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E)
Related products:
Cyclin B1 antibodies; Cyclin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
预测分子量 48 kDa
翻译后修饰 Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

检测图片 (1) Click the Picture to Zoom In

  • ARG62456 anti-Cyclin B1 antibody [V152] WB image

    Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.

克隆号文献

Casein kinase 1δ-dependent Wee1 protein degradation.

WB / Human

Penas C et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line

Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity.

WB / 

Nabti I et al.
J Cell Biol.,  (2014)

publication_link

 

hr_line

Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication.

Luo Y et al.
J Virol.,  (2013)

publication_link

 

hr_line

Tumor dynamics in response to antiangiogenic therapy with oral metronomic topotecan and pazopanib in neuroblastoma xenografts.

IHC-P / Mouse

Kumar S et al.
Transl Oncol.,  (2013)

publication_link

 

hr_line

The role of RING box protein 1 in mouse oocyte meiotic maturation.

Zhou L et al.
PLoS One.,  (2013)

publication_link

 

hr_line

APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing.

WB / 

Holt JE et al.
Mol Biol Cell.,  (2012)

publication_link

 

hr_line

Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.

ICC/IF / 

Hu CK et al.
Mol Biol Cell.,  (2012)

publication_link

 

hr_line

Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.

IHC / Rat

Woo GH et al.
Cancer Sci.,  (2009)

publication_link

 

hr_line

Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.

FACS / Human

Zhang JG et al.
Clin Cancer Res.,  (2007)

publication_link

 

hr_line

Citron kinase is a cell cycle-dependent, nuclear protein required for G2/M transition of hepatocytes.

WB / Rat

Liu H et al.
J Biol Chem.,  (2003)

publication_link

 

hr_line

Kinetics of endomitosis in primary murine megakaryocytes.

FACS / Mouse

Carow CE et al.
J Cell Physiol.,  (2001)

publication_link

 

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