ARG62456
anti-Cyclin B1 antibody [V152]
anti-Cyclin B1 antibody [V152] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [V152] recognizes Cyclin B1 | 
|---|---|
| 反应物种 | Hu | 
| 预测物种 | Ms | 
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB | 
| 特异性 | Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression. | 
| 宿主 | Mouse | 
| 克隆 | Monoclonal | 
| 克隆号 | V152 | 
| 同位型 | IgG1 | 
| 靶点名称 | Cyclin B1 | 
| 抗原物种 | Hamster | 
| 抗原 | His-tagged Hamster Cyclin B1 expressed in bacteria | 
| 偶联标记 | Un-conjugated | 
| 別名 | G2/mitotic-specific cyclin-B1; CCNB | 
应用说明
| 应用建议 | 
									
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | 
属性
| 形式 | Liquid | 
|---|---|
| 纯化 | Protein G purified | 
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide | 
| 抗菌剂 | 0.02% Sodium azide | 
| 浓度 | 0.2 mg/ml | 
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. | 
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. | 
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Ccnb1 | 
| 全名 | cyclin B1 | 
| 背景介绍 | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] | 
| 产品亮点 | Related Antibody Duos and Panels: ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) Related products: Cyclin B1 antibodies; Cyclin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies;  | 
						
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody | 
| 预测分子量 | 48 kDa | 
| 翻译后修饰 | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.  | 
						
检测图片 (1) Click the Picture to Zoom In
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