ARG59155
anti-Cullin 5 antibody
anti-Cullin 5 antibody for Western blot and Human,Mouse,Rat
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cullin 5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cullin 5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-300 of Human Cullin 5 (NP_003469.2). |
| 偶联标记 | Un-conjugated |
| 別名 | VACM-1; CUL-5; VACM1; Vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | PC3 | ||||
| 实际分子量 | 98 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CUL5 |
| 全名 | cullin 5 |
| 生物功能 | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. [UniProt] |
| 预测分子量 | 91 kDa |
| 翻译后修饰 | Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59155 anti-Cullin 5 antibody WB image
Western blot: HEK293T stained with ARG59155 anti-Cullin 5 antibody.
From Li Y et al. Cell Reports (2024), doi: 10.1016/j.celrep.2024.113749, Fig. 5. B.
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ARG59155 anti-Cullin 5 antibody WB image
Western blot: 25 µg of PC3 cell lysate stained with ARG59155 anti-Cullin 5 antibody at 1:1000 dilution.
文献引用
Cullin 3-mediated ubiquitination restricts enterovirus D68 replication and is counteracted by viral protease 3C
ICC / Human
Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition
WB / Human
