ARG58018
anti-Cullin 2 antibody
anti-Cullin 2 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cullin 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cullin 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the middle region of Human Cullin 2. (HECQQRMVADHLQFLHA) |
| 偶联标记 | Un-conjugated |
| 別名 | Cullin-2; CUL-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~87 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CUL2 |
| 全名 | cullin 2 |
| 生物功能 | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt] |
| 预测分子量 | 87 kDa |
| 翻译后修饰 | Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58018 anti-Cullin 2 antibody WB image
Western blot: 1) A431, 2) SMMC-7721, 3) HeLa and 4) COLO320 cell lysates stained with ARG58018 anti-Cullin 2 antibody.
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ARG58018 anti-Cullin 2 antibody WB image
Western blot: Rat brain tissue lysate stained with ARG58018 anti-Cullin 2 antibody.
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ARG58018 anti-Cullin 2 antibody WB image
Western blot: 1) 10 ng, 2) 5 ng and 3) 2.5 ng of recombinant Human protein stained with ARG58018 anti-Cullin 2 antibody.
