ARG63159
anti-Cortactin / EMS1 antibody
anti-Cortactin / EMS1 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Cortactin / EMS1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cortactin / EMS1 |
| 抗原物种 | Human |
| 抗原 | WKASAGHAVSIA-C |
| 偶联标记 | Un-conjugated |
| 別名 | EMS1; Amplaxin; Src substrate cortactin; Oncogene EMS1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding. |
检测图片 (3) Click the Picture to Zoom In
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ARG63159 anti-Cortactin / EMS1 antibody WB image
Western Blot: Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG63159 anti-Cortactin / EMS1 antibody at 0.01 µg/ml dilution.
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ARG63159 anti-Cortactin / EMS1 antibody WB image
Western blot: 35 µg of Human brain (cerebellum) lysate (in RIPA buffer) stained with ARG63159 anti-Cortactin / EMS1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63159 anti-Cortactin / EMS1 antibody WB image
Western blot: 35 µg of HEK293 cell lysate (in RIPA buffer) stained with ARG63159 anti-Cortactin / EMS1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
