ARG10711

anti-Coronin 1 / TACO antibody

anti-Coronin 1 / TACO antibody for ICC/IF,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

概述

产品描述 Rabbit Polyclonal antibody recognizes Coronin 1 / TACO
反应物种 Hu, Ms, Rat, Cow, Hrs, Pig
预测物种 Chk
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Coronin 1 / TACO
抗原 Full length recombinant protein.
偶联标记 Un-conjugated
別名 Clipin-A; Coronin-1A; Coronin-like protein A; CLABP; IMD8; CLIPINA; Tryptophan aspartate-containing coat protein; HCORO1; Coronin-like protein p57; TACO; p57

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:500 - 1:1000
WB1:20000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification.
缓冲液 PBS and 50% Glycerol.
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11151 Human CORO1A

GeneID: 12721 Mouse CORO1A

GeneID: 155151 Rat CORO1A

基因名称 CORO1A
全名 coronin, actin binding protein, 1A
背景介绍 This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
生物功能 May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. [UniProt]
预测分子量 51 kDa
翻译后修饰 phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.
Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.

检测图片 (4) Click the Picture to Zoom In

  • ARG10711 anti-Coronin 1 / TACO antibody ICC/IF image

    Immunocytochemistry: A mixed neuron / glial culture from newborn Rat brain stained with ARG10711 anti-Coronin 1 / TACO antibody (green) at 1:10000 and co-stained with chicken antibody to vimentin at 1:5000. Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

  • ARG10711 anti-Coronin 1 / TACO antibody ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10711 anti-Coronin 1 / TACO antibody (red) at 1:1000 dilution and costained with Mouse mAb to GFAP (green) at 1:1000 dilution. Hoechst (blue) for nuclear staining.

    The Coronin 1 antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.

  • ARG10711 anti-Coronin 1 / TACO antibody WB image

    Western blot: HL60 cell extract stained with ARG10711 anti-Coronin 1 / TACO antibody at 1:10000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa corresponds to Coronin 1a.

  • ARG10711 anti-Coronin 1 / TACO antibody WB image

    Western blot: Mouse brain, Rat brain, Cow cerebellum, Cow cortex and Pig spinal cord lysates stained with ARG10711 anti-Coronin 1 / TACO antibody (green) at 1:5000 dilution.