ARG51744
anti-Connexin 43 phospho (Ser367) antibody
anti-Connexin 43 phospho (Ser367) antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Connexin 43 phospho (Ser367) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Connexin 43 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 367 (R-A-S(p)-S-R) derived from Human Connexin 43. |
| 偶联标记 | Un-conjugated |
| 別名 | Gap junction 43 kDa heart protein; CX43; PPKCA; CMDR; Gap junction alpha-1 protein; HSS; AVSD3; Connexin-43; HLHS1; EKVP; GJAL; ODDD; Cx43 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-Fr: Refer to the reference: PMID: 35110506
for the detailed protocol. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GJA1 |
| 全名 | gap junction protein, alpha 1, 43kDa |
| 背景介绍 | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. |
| 生物功能 | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity). Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. |
检测图片 (3) Click the Picture to Zoom In
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ARG51744 anti-Connexin 43 phospho (Ser367) antibody IHC-Fr image
Immunohistochemistry: Frozen Mouse melanoma stained with ARG51744 anti-Connexin 43 phospho (Ser367) antibody.
From Khatun A et al. Biol Pharm Bull- (2022), doi: 10.1248/bpb.b21-00714, Fig. 2.
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ARG51744 anti-Connexin 43 phospho (Ser367) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with PMA stained with ARG51744 anti-Connexin 43 phospho (Ser367) antibody.
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ARG51744 anti-Connexin 43 phospho (Ser367) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51744 anti-Connexin 43 phospho (Ser367) antibody (left) or the same antibody preincubated with blocking peptide (right).
文献引用
Effective Anticancer Therapy by Combination of Nanoparticles Encapsulating Chemotherapeutic Agents and Weak Electric Current
IHC-Fr / Mouse
