ARG64928
anti-Connexin 43 antibody
anti-Connexin 43 antibody for Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Connexin 43 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Connexin 43 |
| 抗原物种 | Human |
| 抗原 | C-QPFDFPDDNQNSKK |
| 偶联标记 | Un-conjugated |
| 別名 | Gap junction 43 kDa heart protein; CX43; PPKCA; CMDR; Gap junction alpha-1 protein; HSS; AVSD3; Connexin-43; HLHS1; EKVP; GJAL; ODDD; Cx43 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity). Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. |
检测图片 (2) Click the Picture to Zoom In
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ARG64928 anti-Connexin 43 antibody WB image
Western blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG64928 anti-Connexin 43 antibody at 0.3 µg/ml dilution.
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ARG64928 anti-Connexin 43 antibody WB image
Western blot: 35 µg of Human (A) and Rat (B) heart lysates (in RIPA buffer) stained with ARG64928 anti-Connexin 43 antibody at 0.1 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
