ARG10084

anti-Colorectal Carcinoma / CD3 antibody [BS-1]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Mouse Monoclonal antibody [BS-1] recognizes colorectal carcinoma / CD3. This is a bispecific antibody produced by fusion of two hybridoma cell lines. The human colorectal carcinoma antigen (CRC) mAb secreting cell line was transfected by mpSV2gpt. The human CD3 mAb secreting cell line (JXT3) was transfected by mpSV2neo. The somatic fusion between CRC and JXT3 cells produced quadroma CRCgpt / CD3neo, which were selected and cloned in media containing both Mycophenolic acid and Geneticin. Quadromas showing both murine IgG1 and IgG2a was subcloned for bispecific antibody CRC / CD3 production.
反应物种	Hu
应用	ELISA, FACS
宿主	Mouse
克隆	Monoclonal
克隆号	BS-1
同位型	IgG1 / IgG2a
靶点名称	Colorectal Carcinoma / CD3
抗原物种	Human
抗原	The original CRC mAb (Y94) used human colorectal carcinoma as immunogen. The original CD3 mAb (JXT3) used human peripheral T lymphocytes as immunogen
偶联标记	Un-conjugated

应用说明

应用说明	ELISA: The bi-specific antibody has been shown to detect the presence of the tumor-associated antigen in the serum of patients with colorectal carcinoma, and reacted with mucin-like oncofetal pancarcinoma antigen, glycoprotein TAG-72. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Protein G affinity purified
缓冲液	0.01M PBS (pH 7.0)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	Colorectal carcinoma is the cancer developed in the colon or rectum of the digestive system. In developed countries, it is the most common cancer in aging population. Genetic deposition and a less active life style contribute to the development of the cancer. Molecular pathological study showed that altered Wnt-APC-β-catenim signaling pathway, mutated p53, and deactivated TGF-β and DCC (Deleted in Colon Cancer) are involved with the pathogenesis. The cancer is currently screened with a fecal occult blood test in people over 50 years old and the malignancy is confirmed by tumor biopsy. The search for specific biomarker for non-invasive test is still ongoing. CD3 exists on the cell surface of all T-cell types. It is used for differentiating T-cells from other leukocytes such as B cells and natural killer cells. CD3 is the accessory molecule in the T cell receptor complex. In the presence of CD3 and ζ-chain, T-cell receptor binds to antigen presented by MHC and transfers signal for T-cell activation. The hybrid bi-specific antibody binds to CD3 and colorectal carcinoma related antigen at its two different Fabs. Theoretically, the bi-specific antibody brings the target cancer antigen near T-cells and could enhance T-cell mediated immunity to cancer. However, if the binding to CD3 disrupts the CD3’s accessory function, T-cell immunity suppression could be resulted.
研究领域	Controls and Markers antibody; Colorectal Carcinoma Marker antibody

背景介绍

Colorectal carcinoma is the cancer developed in the colon or rectum of the digestive system. In developed countries, it is the most common cancer in aging population. Genetic deposition and a less active life style contribute to the development of the cancer. Molecular pathological study showed that altered Wnt-APC-β-catenim signaling pathway, mutated p53, and deactivated TGF-β and DCC (Deleted in Colon Cancer) are involved with the pathogenesis. The cancer is currently screened with a fecal occult blood test in people over 50 years old and the malignancy is confirmed by tumor biopsy. The search for specific biomarker for non-invasive test is still ongoing. CD3 exists on the cell surface of all T-cell types. It is used for differentiating T-cells from other leukocytes such as B cells and natural killer cells. CD3 is the accessory molecule in the T cell receptor complex. In the presence of CD3 and ζ-chain, T-cell receptor binds to antigen presented by MHC and transfers signal for T-cell activation. The hybrid bi-specific antibody binds to CD3 and colorectal carcinoma related antigen at its two different Fabs. Theoretically, the bi-specific antibody brings the target cancer antigen near T-cells and could enhance T-cell mediated immunity to cancer. However, if the binding to CD3 disrupts the CD3’s accessory function, T-cell immunity suppression could be resulted.

研究领域

Controls and Markers antibody; Colorectal Carcinoma Marker antibody

检测图片 (1) Click the Picture to Zoom In

ARG10084 anti-Colorectal Carcinoma/CD3 antibody [BS-1] Flow Cytometry image

Flow Cytometry: Jurkat cells stained with anti-Colorectal Carcinoma/CD3 antibody [BS-1] (ARG10084) followed by incubation with fluorescence labelled secondary antibody.