ARG67143

anti-Collagen IV antibody

anti-Collagen IV antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Angiogenesis Study antibody; Basement Membrane Marker antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes Collagen IV
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Collagen IV
抗原 Synthetic peptide
偶联标记 Un-conjugated
別名 COL4A1; Collagen Type IV Alpha 1 Chain; Collagen Alpha-1(IV) Chain; Collagen Of Basement Membrane, Alpha-1 Chain; Collagen IV, Alpha-1 Polypeptide; Collagen, Type IV, Alpha 1; COL4A1 NC1 Domain; EC 6.3.1.2; EC 3.4.23; Arresten; COL4A1s; PADMAL; BSVD1; RATOR; BSVD

应用说明

应用建议
应用 推荐稀释比
IHC-P1:300-1:500
WB1:1000-1:1500
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
抗菌剂 0.025% ProClin 300
稳定剂 20% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1282 Human COL4A1

GeneID: 12826 Mouse COL4A1

Swiss-port # P02462 Human Collagen alpha-1(IV) chain

Swiss-port # P02463 Mouse Collagen alpha-1(IV) chain

基因名称 COL4A1
全名 Collagen Type IV Alpha 1 Chain
背景介绍 This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
生物功能 Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. [UniProt]
细胞定位 Basement membrane, Extracellular matrix, Secreted. [UniProt]
研究领域 Angiogenesis Study antibody; Basement Membrane Marker antibody
预测分子量 161 kDa
翻译后修饰 Disulfide bond, Glycoprotein, Hydroxylation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG67143 anti-Collagen IV antibody IHC-P image

    Immunohistochemistry: Human placenta stained with ARG67143 anti-Collagen IV antibody at 1:100 dilution.

  • ARG67143 anti-Collagen IV antibody WB image

    Western blot: Human placenta stained with ARG67143 anti-Collagen IV antibody.

  • ARG67143 anti-Collagen IV antibody IHC-P image

    Immunohistochemistry: Mouse liver stained with ARG67143 anti-Collagen IV antibody at 1:100 dilution.