ARG20786

anti-Collagen III antibody, pre-adsorbed

anti-Collagen III antibody, pre-adsorbed for ELISA,Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Hamster,Pig,Rabbit

publication_link 参考文献6

概述

产品描述

Goat Polyclonal antibody recognizes Collagen III

反应物种 Hu, Ms, Rat, Bov, Chk, Dog, Hm, Pig, Rb
应用 ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB
特异性 The antibody reacts with conformational determinants on type III collagen. The antibody is pre-adsorbed with Collagen types I, II, IV, V and VI, so the antibody may not react with Collagen types I, II, IV, V and VI.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Collagen III
抗原物种 Human
抗原 Human Type III collagen
偶联标记 Un-conjugated
別名 EDS4A; Collagen alpha-1(III) chain

应用说明

预吸附 Collagen types I, II, IV, V and VI.
应用建议
应用 推荐稀释比
ELISAAssay-dependent
EMAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1:100 - 1:1000
应用说明 WB: The antibody reacts with native and denature form of Collagen III protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 BBS (pH 8.2)
浓度 0.4 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1281 Human COL3A1

GeneID: 12825 Mouse COL3A1

GeneID: 84032 Rat COL3A1

基因名称 COL3A1
全名 collagen, type III, alpha 1
背景介绍 This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
生物功能 Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt]
预测分子量 139 kDa
翻译后修饰 Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

检测图片 (4) Click the Picture to Zoom In

  • ARG20786 anti-Collagen III antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Rabbit bone marrow stromal cells stained with ARG20786 anti-Collagen III antibody, pre-adsorbed.

    From Cheng-Chang Lu et al. Bone Joint Res. (2023), doi: 10.1302/2046-3758.121.BJR-2021-0434.R2, Fig. 6.

  • ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG20786 anti-Collagen III antibody, pre-adsorbed WB image

    Western blot: Embryonic chick cardiac tissue samples separated by SDS-PAGE and stained with ARG20786 anti-Collagen III antibody (pre-adsorbed) under reducing conditions.

  • ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

文献引用

Anterior cruciate ligament remnant preservation attenuates apoptosis and enhances the regeneration of hamstring tendon graft

ICC/IF / Rabbit

Cheng-Chang Lu et al.
Bone Joint Res.,  (2023)

publication_link

 

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Vinpocetine Ameliorates Metabolic-Syndrome-Associated Bladder Overactivity in Fructose-Fed Rats by Restoring Succinate-Modulated cAMP Levels and Exerting Anti-Inflammatory Effects in the Bladder Detrusor Muscle

WB / Mouse

Wei-Chia Lee et al.
Biomedicines,  (2022)

publication_link

 

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Comparative therapeutic strategies for preventing aortic rupture in a mouse model of vascular Ehlers-Danlos syndrome

IHC-P / Mouse

Anne Legrand et al.
PLoS Genet,  (2022)

publication_link

 

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Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea.

WB / Rabbit

Paramananda Saikia et al.
Sci Rep.,  (2020)

publication_link

 

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Extracorporeal shock wave promotes activation of anterior cruciate ligament remnant cells and their paracrine regulation of bone marrow stromal cells' proliferation, migration, collagen synthesis, and differentiation.

ICC/IF / Human

Cheng-Chang Lu et al.
Bone Joint Res.,  (2020)

publication_link

 

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Ba-Wei-Die-Huang-Wan (Hachimi-jio-gan) can ameliorate ketamine-induced cystitis by modulating neuroreceptors, inflammatory mediators, and fibrogenesis in a rat model.

WB / Rat

Lee Wei-Chia et al.
Neurourol Urodyn.,  (2019)

publication_link

 

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