ARG20787

anti-Collagen II antibody, pre-adsorbed

anti-Collagen II antibody, pre-adsorbed for ELISA,Electron microscopy,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Pig,Rabbit,Sheep

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概述

产品描述

Goat Polyclonal antibody recognizes Collagen II

反应物种 Hu, Ms, Rat, Bov, Chk, Pig, Rb, Sheep
应用 ELISA, EM, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性 The antibody reacts with conformational determinants on type II collagen. The antibody is pre-adsorbed with Collagen types I, III, IV, V and VI, so the antibody may not react with Collagen types I, III, IV, V and VI.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Collagen II
抗原物种 Human
抗原 Collagen II
偶联标记 Un-conjugated
別名 AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen)

应用说明

预吸附 Collagen types I, III, IV, V and VI.
应用建议
应用 推荐稀释比
ELISA1:1,000 – 1:4,000
EMAssay-dependent
FLISA1:200 – 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:100 – 1:300
IPAssay-dependent
WB1:100-1:300
应用说明 WB: The antibody reacts with native and denature form of Collagen II protein.
IHC-P: Antigen Retrieval: treat sections with 2 µg/ml proteinase K at 37°C for 15 min before staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 BBS (pH 8.2)
浓度 0.4 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1280 Human COL2A1

GeneID: 12824 Mouse COL2A1

GeneID: 25412 Rat COL2A1

基因名称 COL2A1
全名 collagen, type II, alpha 1
背景介绍 This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
生物功能 Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt]
预测分子量 142 kDa
翻译后修饰 Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.

检测图片 (4) Click the Picture to Zoom In

  • ARG20787 anti-Collagen II antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Porcine primary chondrocytes fixed with 4% Paraformaldehyde for 10 min at RT and blocked by 1% BSA, 0.1% Triton for 30 min at RT. Cells were incubated in 5% BSA and stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) at 1:100 for 3 hours at RT.

  • ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse rib tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) followed by Rabbit anti-Goat IgG (H+L) antibody (FITC) and DAPI.

  • ARG20787 anti-Collagen II antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded tibiae section from E15 mouse was stained with ARG20787 anti-Collagen II antibody (pre-adsorbed).

  • ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image

    Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) (right) followed by Donkey anti-Goat IgG (H+L) antibody (Alexa Fluor® 555) and DAPI.

客户反馈

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anti-Collagen II antibody, pre-adsorbed

Application:IF/ICC

Sample:Porcine primary chondrocytes

Fixation Buffer:4% Paraformaldehyde

Fixation Time:10 min

Fixation Temperature:RT ºC

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:3 hours

Primary Antibody Incubation Temperature:RT ºC

文献引用

Nanohydroxyapatite Hydrogel Can Promote the Proliferation and Migration of Chondrocytes and Better Repair Talar Articular Cartilage

IF / Mouse

Yuxuan Zhang et al.
Computational and Mathematical Methods in Medicine ,  (2022)

publication_link

 

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Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway

WB / Pig

Zi-Miao Liu et al.
Bone Joint Res.,  (2021)

publication_link

 

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Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration.

IHC-P / Rabbit

Pei Liu et al.
Chemical Engineering Journal.,  (2021)

publication_link

 

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In situ self imine-crosslinked nanocomplexes loaded with small noncoding RNA for efficient osteoarthritis attenuation.

IHC-P / Mouse

Yu Zhu et al.
Chemical Engineering Journal.,  (2020)

publication_link

 

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Bioinspired stratified electrowritten fiber-reinforced hydrogel constructs with layer-specific induction capacity for functional osteochondral regeneration.

ICC/IF, IHC-P / Rabbit

Zhiguang Qiao et al.
Biomaterials.,  (2020)

publication_link

 

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Shockwave Treatment Enhances the Extracellular Matrix Production in Articular Chondrocyte Through Activation of the ROS/MAPK/Nrf2 Signaling Pathway.

WB / Pig

Shen Po-Chih et al.
Preprint.,  (2020)

publication_link

 

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A Decellularized Scaffold Derived From Squid Cranial Cartilage for Use in Cartilage Tissue Engineering.

IHC-P / Squid

Lim Thou et al.
J Mater Chem B.,  (2020)

publication_link

 

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A novel elastic and controlled-release poly(ether-ester-urethane)urea scaffold for cartilage regeneration.

IHC-P / Rabbit

Chen Chang'an et al.
J Mater Chem B.,  (2020)

publication_link

 

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