ARG62450
anti-Collagen II antibody [5B2.5]
anti-Collagen II antibody [5B2.5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Chicken,Human,Mouse,Pig,Rabbit
Developmental Biology antibody; Signaling Transduction antibody
参考文献3
概述
| 产品描述 | Mouse Monoclonal antibody [5B2.5] recognizes Collagen II |
|---|---|
| 反应物种 | Hu, Ms, Chk, Pig, Rb |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5B2.5 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Collagen II |
| 抗原物种 | Chicken |
| 抗原 | A purified preparation of lathyritic type II collagen from embryonic chicken sternum |
| 偶联标记 | Un-conjugated |
| 別名 | Collagen alpha-1(II) chain; Alpha-1 type II collagen |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified |
| 缓冲液 | 1X PBS buffer with 0.1% sodium azide. |
| 抗菌剂 | 0.1% sodium azide. |
| 浓度 | 2 mg/ml |
| 存放说明 | Aliquot and store at 2-8°C. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COL2A1 |
| 全名 | collagen, type II, alpha 1 |
| 背景介绍 | The alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [provided by Uniprot] |
| 细胞定位 | Secreted |
| 研究领域 | Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 142 kDa |
| 翻译后修饰 | Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. |
检测图片 (3) Click the Picture to Zoom In
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ARG62450 anti-Collagen II antibody [5B2.5] IHC-P image
Immunohistochemistry: Rat condylar cartilage stained with ARG62450 anti-Collagen II antibody [5B2.5].
From Cai W et al. Mol Med. (2025), doi: 10.1186/s10020-025-01186-6, Fig. 2. D.
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ARG62450 anti-Collagen II antibody [5B2.5] ICC/IF image
Immunofluorescence: Rabbit NPSC stained with ARG62450 anti-Collagen II antibody [5B2.5].
From Shan-Zheng Wang et al. Stem Cell Res Ther. (2018), doi: 10.1186/s13287-018-0937-7, Fig. 6I.
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ARG62450 anti-Collagen II antibody [5B2.5] WB image
Western blot: Rabbit NPSC stained with ARG62450 anti-Collagen II antibody [5B2.5].
From Wang SZ et al. Stem Cell Res Ther- (2018), doi: 10.1186/s13287-018-0937-7, Fig. 6. H.
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