ARG21965

anti-Collagen I antibody, pre-adsorbed

anti-Collagen I antibody, pre-adsorbed for Electron microscopy,ELISA,FLISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Bovine,Cat,Chicken,Dog,Elephant,Guinea pig,Hamster,Human,Mouse,Pig,Rabbit,Rat,Sheep

publication_link 参考文献21

概述

产品描述

Goat Polyclonal antibody recognizes Collagen I

反应物种 Hu, Ms, Rat, Bov, Cat, Chk, Dog, Elp, Gpig, Hm, Pig, Rb, Sheep
应用 ELISA, EM, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性 The antibody reacts with conformational determinants on type I collagen. The antibody is pre-adsorbed with Collagen types II, III, IV, V and VI, so the antibody may not react with Collagen types II, III, IV, V and VI.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Collagen I
抗原物种 Human
抗原 Human Type I Collagen
偶联标记 Un-conjugated
別名 collagen type I alpha 1 chain; OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen; Collagen type I alpha 2 chain; Collagen alpha-2(I) chain

应用说明

预吸附 Collagen types II, III, IV, V and VI.
应用建议
应用 推荐稀释比
ELISA1:1,000 – 1:4,000
EMAssay-dependent
FACSAssay-dependent
FLISA1:200 – 1:400
ICC/IF1:40 – 1:1,000
IHC-Fr1:10 - 1:40
IHC-P1:10 - 1:40
IPAssay-dependent
WB1:200 – 1:1,000
应用说明 WB: The antibody reacts with native and denature form of Collagen I protein.
IHC-P: Antigen retrieval: select one of below:
1. Digestion with 0.1% pepsin from porcine gastric mucosa 3200–4500 units per mg (Sigma, Vienna, Austria) in 0.5 M acetic acid for 2 h at 37°C.
2. Microwave for 4 X 5 min at 800W in 0.01 M Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~120-190 kDa (depending on the sample types)

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 BBS (pH 8.2)
浓度 0.4 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 COL1A1; COL1A2
全名 collagen, type I
背景介绍 Collagen type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
生物功能 Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30346 Myofibroblast / Fibrosis Antibody Panel
Related products:
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
Collagen I antibody for studying rabbit bone differentiation
New antibody panels for Myofibroblasts and CAFs
预测分子量 COL1A1: 139 kDa
COL1A2: 129 kDa
翻译后修饰 Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

检测图片 (7) Click the Picture to Zoom In

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Rabbit osteochondral stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:100 dilution.

    From Wenli Dai et al. Enhanced osteochondral repair with hyaline cartilage formation using an extracellular matrix-inspired natural scaffold (2023), doi: 10.1016/j.scib.2023.07.050, Fig. 7a.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Rabbit synovium-derived mesenchymal stem cell stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:1000 dilution.

    From Zong Li e et al. Chemical Engineering Journal, (2023), doi: 10.1016/j.cej.2023.145209, Fig. 4B.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Canine aorta mesothelial stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:500 dilution.

    From Masakazu Shimada et al. PLoS One. (2022), doi: 35061786, Fig. 1.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Human fibroblasts were stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-Fr image

    Immunohistochemistry: Frozen MC4R-KO Mouse liver section was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed), anti-F4/80 antibody followed by secondary antibodies and DAPI.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed WB image

    Western blot: Purified Human Type I Collagen stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin embedded Rat kidney section post uninephrectomy was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed) followed by a secondary antibody and AEC.

文献引用

Effect of lyophilized exosomes derived from umbilical cord stem cells on chronic anterior cruciate ligament cell injury

ICC/IF / Human

Hon-Lok Lo et al.
Preprint,  (2024)

publication_link

 

hr_line

Mesenchymal Stem Cells Preconditioned with Hypoxia and Dexamethasone Promote Osteoblast Differentiation Under Stress Conditions

ICC/IF / Human

Miyako Shimasaki et al.
Journal of Medical Sciences,  (2024)

publication_link

 

hr_line

Resveratrol attenuates inflammation and fibrosis in rheumatoid arthritis-associated interstitial lung disease via the AKT/TMEM175 pathway

WB, IHC-P / Human, Mouse

Nannan Liu et al.
J Transl Med.,  (2024)

publication_link

 

hr_line

Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells

ICC/IF / Rabbit

Mingze Du et al.
Bioact Mater,  (2024)

publication_link

 

hr_line

Ultrasound-Controlled Delivery of Growth Factor-Loaded Cerasomes Combined with Polycaprolactone Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells for Biomimetic Tendon-to-Bone Interface Engineering

IHC-P / Rabbit

Cancan Du et al.
ACS Appl Mater Interfaces.,  (2024)

publication_link

 

hr_line

Anti-Apoptosis Therapy for Meniscal Avascular Zone Repair: A Proof-of-Concept Study in a Lapine Model

IHC-P / Rabbit

Wenqiang Yan et al.
Bioengineering (Basel).,  (2023)

publication_link

 

hr_line

Enhanced osteochondral repair with hyaline cartilage formation using an extracellular matrix-inspired natural scaffold

IHC-P / Rabbit

Wenli Dai et al.
Sci Bull (Beijing).,  (2023)

publication_link

 

hr_line

Regional specific tunable meniscus decellularized extracellular matrix (MdECM) reinforced bioink promotes anistropic meniscus regeneration

ICC/IF / Rabbit

Zong Li et al.
Chemical Engineering Journal,  (2023)

publication_link

 

hr_line

Anterior cruciate ligament remnant preservation attenuates apoptosis and enhances the regeneration of hamstring tendon graft

ICC/IF / Rabbit

Cheng-Chang Lu et al.
Bone Joint Res.,  (2023)

publication_link

 

hr_line

The transplantation of particulated juvenile allograft cartilage and synovium for the repair of meniscal defect in a lapine model

IHC-P / Rabbit

Wenqiang Yan et al.
J Orthop Translat.,  (2022)

publication_link

 

hr_line

Effects of long-term and high-dose administration of glucocorticoids on the cranial cruciate ligament in healthy beagle dogs

IHC-P / Dog

Masakazu Shimada et al.
PLoS One.,  (2022)

publication_link

 

hr_line

Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway

WB / Mouse

Lanxin Bao et al.
Molecules,  (2022)

publication_link

 

hr_line

A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis

ICC/IF / Human

Pengwei Deng et al.
Bioengineering (Basel),  (2022)

publication_link

 

hr_line

Loss of MLKL ameliorates liver fibrosis by inhibiting hepatocyte necroptosis and hepatic stellate cell activation

IHC-P / Mouse

Ren Guo et al.
Theranostics,  (2022)

publication_link

 

hr_line

Vinpocetine Ameliorates Metabolic-Syndrome-Associated Bladder Overactivity in Fructose-Fed Rats by Restoring Succinate-Modulated cAMP Levels and Exerting Anti-Inflammatory Effects in the Bladder Detrusor Muscle

WB / Mouse

Wei-Chia Lee et al.
Biomedicines,  (2022)

publication_link

 

hr_line

Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration.

IHC-P / Rabbit

Pei Liu et al.
Chemical Engineering Journal.,  (2021)

publication_link

 

hr_line

hDPSC-laden GelMA microspheres fabricated using electrostatic microdroplet method for endodontic regeneration.

ICC/IF / Human

Ting Yang et al.
Mater Sci Eng C Mater Biol Appl.,  (2021)

publication_link

 

hr_line

Effect of Freshly Isolated Bone Marrow Mononuclear Cells and Cultured Bone Marrow Stromal Cells in Graft Cell Repopulation and Tendon-Bone Healing after Allograft Anterior Cruciate Ligament Reconstruction.

IHC-P / Rabbit

Cheng-Chang Lu et al.
Int J Mol Sci.,  (2021)

publication_link

 

hr_line

Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration.

WB / Human

Yang Xueting et al.
Theranostics.,  (2019)

publication_link

 

hr_line

Ba-Wei-Die-Huang-Wan (Hachimi-jio-gan) can ameliorate ketamine-induced cystitis by modulating neuroreceptors, inflammatory mediators, and fibrogenesis in a rat model.

WB / Rat

Lee Wei‐Chia et al.
Neurourology and Urodynamics.,  (2019)

publication_link

 

hr_line

Activation of mTORC1 in fibroblasts accelerates wound healing and induces fibrosis in mice.

WB / Mouse

Hu Xiao et al.
Wound Repair Regen.,  (2019)

publication_link

 

hr_line