ARG52247

anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody

anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody
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概述

产品描述 Rabbit Polyclonal antibody recognizes Collagen I, alpha 1 propeptide (Procollagen I)
反应物种 Hu, Ms, Rat
预测物种 Amph, Bird, Fsh, Mamm
应用 ICC/IF, IHC-P, WB
特异性 This antibody recognizes pro-collagen I, but not mature form of collagen I.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Pro-Collagen I
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to amino acid residues specific to the collagen I, alpha 1 propeptide.
偶联标记 Un-conjugated
別名 OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IHC-P1:100
WB1:1,000
应用说明 Specific for the propeptide portion of the ~180 kDa collagen I α1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human lung; Mouse lung; Rat lung
实际分子量 180- 210 kDa

属性

形式 Liquid
纯化 Affinity Purified
缓冲液 PBS
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1277 Human COL1A1

GeneID: 12842 Mouse COL1A1

GeneID: 29393 Rat COL1A1

基因名称 COL1A1
全名 collagen, type I, alpha 1
背景介绍 Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).
产品亮点 Related products:
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
研究领域 Signaling Transduction antibody
预测分子量 139 kDa
翻译后修饰 Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

检测图片 (3) Click the Picture to Zoom In

  • ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody ICC/IF image

    Immunofluorescence: Rat bone marrow mesenchymal stem cell stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody.

    From Jiahao Chen et al. IOPscience (2023), doi: 10.1088/1748-605X/ad0476, Fig. 4f.

  • ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody WB image

    Western blot: 30 µg of Rat lung lysate stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody at 1:1000 dilution.

  • ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody IHC image

    Immunohistochemistry: Fibrotic Mouse lung tissue stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.

客户反馈

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anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody

Application:WB

Sample:Rat lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

An HA/PEEK scaffold with modified crystallinity via 3D-bioprinting for multiple applications in hard tissue engineering

ICC/IF / Rat

Jiahao Chen et al.
IOPscience,  (2023)

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Xenoextracellular matrix-rosiglitazone complex-mediated immune evasion promotes xenogenic bioengineered root regeneration by altering M1/M2 macrophage polarization

IHC-P / Rat

Tingting Lan et al.
Biomaterials.,  (2021)

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Treated dentin matrix particles combined with dental follicle cell sheet stimulate periodontal regeneration.

WB / Human

Yang Hefeng et al.
Dent Mater.,  (2019)

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