anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody
anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody
|产品描述||Rabbit Polyclonal antibody recognizes Collagen I, alpha 1 propeptide (Procollagen I)|
|反应物种||Hu, Ms, Rat|
|预测物种||Amph, Bird, Fsh, Mamm|
|特异性||This antibody recognizes pro-collagen I, but not mature form of collagen I.|
|抗原||KLH-conjugated synthetic peptide corresponding to amino acid residues specific to the collagen I, alpha 1 propeptide.|
|別名||OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen|
|应用说明||Specific for the propeptide portion of the ~180 kDa collagen I α1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|阳性对照||Human lung; Mouse lung; Rat lung|
|实际分子量||180- 210 kDa|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||collagen, type I, alpha 1|
|背景介绍||Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).|
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies;
New antibody panels for Myofibroblasts and CAFs
|研究领域||Signaling Transduction antibody|
|翻译后修饰||Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
检测图片 (2) Click the Picture to Zoom In
ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody WB image
Western blot: 30 µg of Rat lung lysate stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody at 1:1000 dilution.
ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody IHC image
Immunohistochemistry: Fibrotic Mouse lung tissue stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.