ARG56973
anti-Cofilin antibody [1C1]
anti-Cofilin antibody [1C1] for ELISA,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1C1] recognizes Cofilin |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1C1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Cofilin |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-166 of Human Cofilin. |
| 偶联标记 | Un-conjugated |
| 別名 | CFL; 18 kDa phosphoprotein; Cofilin, non-muscle isoform; HEL-S-15; p18; cofilin; Cofilin-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CFL1 |
| 全名 | cofilin 1 (non-muscle) |
| 背景介绍 | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
| 生物功能 | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2. |
检测图片 (1) Click the Picture to Zoom In
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ARG56973 anti-Cofilin antibody [1C1] WB image
Western blot: 20 µg of HeLa stained with ARG56973 anti-Cofilin antibody [1C1] at 1:1000.
