ARG63822
anti-Cofilin 2 antibody
anti-Cofilin 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Cofilin 2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cofilin 2 |
| 抗原物种 | Human |
| 抗原 | C-DIKDRSTLGEK |
| 偶联标记 | Un-conjugated |
| 別名 | Cofilin, muscle isoform; NEM7; Cofilin-2 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. |
检测图片 (4) Click the Picture to Zoom In
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ARG63822 anti-Cofilin 2 antibody WB image
Western Blot: Human Skeletal Muscle lysate (35 µg protein in RIPA buffer) stained with ARG63822 anti-Cofilin 2 antibody at 0.003 µg/ml dilution.
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ARG63822 anti-Cofilin 2 antibody WB image
Western Blot: Rat Skeletal Muscle lysate (35 µg protein in RIPA buffer) stained with ARG63822 anti-Cofilin 2 antibody at 0.001 µg/ml dilution.
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ARG63822 anti-Cofilin 2 antibody WB image
Western Blot: 1). Mock transfection; 2) Human Muscle Cofilin 2 (RC222215) expressing plasmid transfected HEK293 cell lysate standed with ARG63822 anti-Cofilin 2 antibody
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ARG63822 anti-Cofilin 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63822 anti-Cofilin 2 antibody at 3.75 µg/ml dilution followed by AP-staining.
