ARG66486
anti-Claudin 3 antibody
anti-Claudin 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Claudin 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1, kappa |
| 靶点名称 | Claudin 3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human Claudin 3. |
| 偶联标记 | Un-conjugated |
| 別名 | CPE-R2; CPE-receptor 2; RVP1; HRVP1; CPE-R 2; CPETR2; hRVP1; C7orf1; Clostridium perfringens enterotoxin receptor 2; Rat ventral prostate.1 protein homolog; Claudin-3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citric acid buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 22 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLDN3 |
| 全名 | claudin 3 |
| 背景介绍 | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. [UniProt] |
| 细胞定位 | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 23 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG66486 anti-Claudin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon stained with ARG66486 anti-Claudin 3 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
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ARG66486 anti-Claudin 3 antibody WB image
Western blot: 30 µg of MCF7 whole cell lysate stained with ARG66486 anti-Claudin 3 antibody at 1:1000 dilution.
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ARG66486 anti-Claudin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestine stained with ARG66486 anti-Claudin 3 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
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ARG66486 anti-Claudin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectum stained with ARG66486 anti-Claudin 3 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
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ARG66486 anti-Claudin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG66486 anti-Claudin 3 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
