anti-Chromogranin A antibody [SQab20205]

anti-Chromogranin A antibody [SQab20205] for IHC-Formalin-fixed paraffin-embedded sections and Human


产品描述 Recombinant Rabbit Monoclonal antibody [SQab20205] recognizes Chromogranin A
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab20205
同位型 IgG
靶点名称 Chromogranin A
抗原物种 Human
抗原 Synthetic peptide within aa. 400-600 of Human Chromogranin A.
偶联标记 Un-conjugated
別名 Vasostatin II; CGA; Vasostatin I; Pituitary secretory protein I; SP-I; CgA; SL21; Chromogranin-A


应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Colon tissue.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 1113 Human CHGA

Swiss-port # P10645 Human Chromogranin-A

基因名称 CHGA
全名 chromogranin A
背景介绍 The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
生物功能 [Pancreastatin]: Strongly inhibits glucose induced insulin release from the pancreas.

[Catestatin]: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).

[Serpinin]: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. [UniProt]
细胞定位 Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. Serpinin: Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Pyroglutaminated serpinin localizes to secretory vesicle. [UniProt]
预测分子量 51 kDa
翻译后修饰 Sulfated on tyrosine residues and/or contains sulfated glycans.

O-glycosylated with core 1 or possibly core 8 glycans.

Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66772 anti-Chromogranin A antibody [SQab20205] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66772 anti-Chromogranin A antibody [SQab20205] at 18°C - 25°C for 30 minutes.