ARG45725
anti-Cathepsin F antibody
anti-Cathepsin F antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cathepsin F |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cathepsin F |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to middle region of human Cathepsin F. |
| 偶联标记 | Un-conjugated |
| 別名 | CTSF; CLN13; EC 3.4.22.41; Cathepsin F; CATSF |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 53 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTSF |
| 全名 | Cathepsin F |
| 背景介绍 | Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008] |
| 生物功能 | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Disulfide bond; Glycoprotein; Zymogen. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45725 anti-Cathepsin F antibody IHC-P image
Immunohistochemistry: Human stomach cancer stained with ARG45725 anti-Cathepsin F antibody at 2 μg/ml dilution.
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ARG45725 anti-Cathepsin F antibody WB image
Western blot: 293T stained with ARG45725 anti-Cathepsin F antibody at 0.5 μg/ml dilution.
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ARG45725 anti-Cathepsin F antibody FACS image
Flow Cytometry: SiHa stained with ARG45725 anti-Cathepsin F antibody at 1 µg/10^6 cells dilution.
