ARG41157
anti-Cathepsin D antibody
anti-Cathepsin D antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Monkey
概述
| 产品描述 | Goat Polyclonal antibody recognizes Cathepsin D |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cathepsin D |
| 抗原物种 | Human |
| 抗原 | Purified recombinant peptide within aa. 275 to the C-terminus of Human Cathepsin D. |
| 偶联标记 | Un-conjugated |
| 別名 | CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y), Dog (MDCK) and Monkey (COS-7) whole cell lysates. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTSD |
| 全名 | cathepsin D |
| 背景介绍 | This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt] |
| 细胞定位 | Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034). [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG41157 anti-Cathepsin D antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells were fixed with methanol and permeabilized with 0.1% saponin. Cells were stained with ARG41157 anti-Cathepsin D antibody at 1:100 dilution.
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ARG41157 anti-Cathepsin D antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue stained with ARG41157 anti-Cathepsin D antibody at 1:200 dilution.
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ARG41157 anti-Cathepsin D antibody WB image
Western blot: 100 ug of MDCK cell lysate and 30 ug of transfected 293FT cell lysate stained with ARG41157 anti-Cathepsin D antibody at 1:500 dilution.
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ARG41157 anti-Cathepsin D antibody EM image
Electron microscopy: Immunogold labeling using melanocytes and ARG41157 anti-Cathepsin D antibody.
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ARG41157 anti-Cathepsin D antibody ICC/IF image
Immunofluorescence: Raw264.7 cells were fixed with PFA and permeabilized with 0.05% saponin. Cells were stained with ARG41157 anti-Cathepsin D antibody (red) at 1:100 dilution. LAMP2 staining (green). nuclear staining (blue).
