ARG66888

anti-Caspase 3 (cleaved) antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μg

399
258

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody recognizes Caspase 3 (cleaved)
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
同位型	IgG1
靶点名称	Caspase 3 (cleaved)
抗原物种	Human
抗原	Recombinant protein of cleaved Caspase 3.
偶联标记	Un-conjugated
別名	CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:50 - 1:200
IHC-P	1:100 - 1:200
WB	1:250 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

HeLa, NIH/3T3 and Rat brain

实际分子量

~ 20 kDa (cleaved form)

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol and 0.5% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 12367 Mouse CASP3 GeneID: 25402 Rat CASP3 GeneID: 836 Human CASP3 Swiss-port # P42574 Human Caspase-3 Swiss-port # P55213 Rat Caspase-3 Swiss-port # P70677 Mouse Caspase-3 More
基因名称	CASP3
全名	caspase 3, apoptosis-related cysteine peptidase
背景介绍	The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
生物功能	Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt]
细胞定位	Cytoplasm. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; Anti-Mouse IgG secondary antibodies;
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
预测分子量	32 kDa
翻译后修饰	Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt]

检测图片 (6) Click the Picture to Zoom In

ARG66888 anti-Caspase 3 (cleaved) antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG66888 anti-Caspase 3 (cleaved) antibody (green) at 1:200 dilution and co-stained with ARG51617 anti-FOXO1 / FKHR phospho (Ser256) antibody (red) at 1:200 dilution, overnight at 4°C.
ARG66888 anti-Caspase 3 (cleaved) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66888 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Negative control was used by secondary antibody only.
ARG66888 anti-Caspase 3 (cleaved) antibody WB image

Western blot: HeLa, NIH/3T3 and Rat brain lysates stained with ARG66888 anti-Caspase 3 (cleaved) antibody.
ARG66888 anti-Caspase 3 (cleaved) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat lung tissue stained with ARG66888 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Negative control was used by secondary antibody only.
ARG66888 anti-Caspase 3 (cleaved) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66888 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Negative control was used by secondary antibody only.
ARG66888 anti-Caspase 3 (cleaved) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG66888 anti-Caspase 3 (cleaved) antibody.