ARG66328
anti-Caspase 3 antibody [SQab1876]
anti-Caspase 3 antibody [SQab1876] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1876] recognizes Caspase 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1876 |
| 同位型 | IgG |
| 靶点名称 | Caspase 3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 1-100 of Human Caspase 3. |
| 偶联标记 | Un-conjugated |
| 別名 | CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP3 |
| 全名 | caspase 3, apoptosis-related cysteine peptidase |
| 背景介绍 | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 32 kDa, 17 kDa (cleaved) |
| 翻译后修饰 | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66328 anti-Caspase 3 antibody [SQab1876] ICC/IF image
Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66328 anti-Caspase 3 antibody [SQab1876] FACS image
Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66328 anti-Caspase 3 antibody [SQab1876] WB image
Western blot: 20 µg of Jurkat treated with 1 µM staurosporine for 4h (+) or untreated (-). The blots were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] at 1:2000 dilution.
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ARG66328 anti-Caspase 3 antibody [SQab1876] IP image
Immunoprecipitation: 0.4 mg of Jurkat whole cell lysate immunoprecipitated (1:50) and stained with ARG66328 anti-Caspase 3 antibody [SQab1876]. 1) ARG66328 IP in Jurkat whole cell lysate, 2) PBS instead of ARG66328 in Jurkat whole cell lysate, and 3) Jurkat whole cell lysate, 10 µg (input).
