ARG20001
anti-Caspase 3 antibody [C33]
anti-Caspase 3 antibody [C33] for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody [C33] recognizes Caspase 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | C33 |
| 同位型 | IgG1 |
| 靶点名称 | Caspase 3 |
| 抗原物种 | Human |
| 抗原 | Recombinant human Caspase-3 |
| 偶联标记 | Un-conjugated |
| 別名 | CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified Antibody |
| 缓冲液 | PBS, 50% Glycerol, 0.5 mg/ml BSA and 0.01% Thimerosal |
| 抗菌剂 | 0.01% Thimerosal |
| 稳定剂 | 50% Glycerol, 0.5 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP3 |
| 全名 | caspase 3, apoptosis-related cysteine peptidase |
| 背景介绍 | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| 生物功能 | Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.
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ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.
客户反馈
Good
anti-Caspase 3 antibody [C33]
Application：WB
Sample：HeLa untreated / treated with Staurosporine (1μM, overnight)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Caspase 3 antibody [C33]
Application：WB
Sample：HeLa without/with Staurosporine
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Caspase 3 antibody [C33]
Application：WB
Sample：PC-3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：4 µg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Moonlighting glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus gasseri inhibits keratinocyte apoptosis and skin inflammation in experimental atopic dermatitis
WB / Mouse
Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.
WB / Mouse (Since arigo failed mouse sample testing, therefore, the specie used in this paper is not covered the arigo's guarantee.)
