ARG10734
anti-Cas9 (S. Pyogenes) antibody
anti-Cas9 (S. Pyogenes) antibody for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cas9 (S. Pyogenes) |
|---|---|
| 反应物种 | Bacteria |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cas9 (S. Pyogenes) |
| 抗原物种 | Bacteria |
| 抗原 | N-terminal region, aa. 1-608 and C-terminal region, aa. 814-1372 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 产品亮点 | Related products: Cas9 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer |
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检测图片 (3) Click the Picture to Zoom In
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ARG10734 anti-Cas9 (S. Pyogenes) antibody ICC/IF image
Immunocytochemistry: Hek293 cell culture transfected with a construct including the C-terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (red) and also with a chicken antibody to S. pyogenes Cas9 (green). A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.
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ARG10734 anti-Cas9 (S. Pyogenes) antibody WB image
Western blot: 1) 100 ng, and 2) 10 ng of full length Cas9 protein from Streptococcus pyogenes was stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody at 1:2000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.
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ARG10734 anti-Cas9 (S. Pyogenes) antibody WB image
Western blot: 1) HEK293 (non-transfected), 2) transfected HEK293 expressing the C-terminal aa. 814-1372 of Cas9 from S. pyogenes, 3) transfected HEK293 expressing the N-terminal 608 amino acids of Cas9 from S. pyogenes and 4) 40 ng of full length S. pyogenes Cas9 protein. The blots were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (green) at 1: 1000 dilution.
