ARG66033

anti-Cardiotrophin 1 antibody

anti-Cardiotrophin 1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes Cardiotrophin 1
反应物种 Hu
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Cardiotrophin 1
抗原物种 Human
抗原 E. coli derived recombinant Human Cardiotrophin 1.
(SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA)
偶联标记 Un-conjugated
別名 CT-1; CT1; Cardiotrophin-1

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66034 as a detection antibody
IHC-P0.25 µg/ml
Neut0.25 - 0.40 µg/ml (To yield [ND50] of the biological activity of hCardiotrophin-1 (15 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1489 Human CTF1

Swiss-port # Q16619 Human Cardiotrophin-1

基因名称 CTF1
全名 cardiotrophin 1
背景介绍 The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
生物功能 Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. [UniProt]
预测分子量 21 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG66033 anti-Cardiotrophin 1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG66033 anti-Cardiotrophin 1 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG66033 anti-Cardiotrophin 1 antibody standard curve image

    Sandwich ELISA: ARG66033 anti-Cardiotrophin 1 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66034 anti-Cardiotrophin 1 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.