ARG10680
anti-Calretinin antibody
anti-Calretinin antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow
概述
| 产品描述 | Chicken Polyclonal antibody recognizes Calretinin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | Calretinin |
| 抗原物种 | Human |
| 抗原 | Full-length recombinant Human Calretinin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | CAB29; CR; CAL2; 29 kDa calbindin; Calretinin |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CALB2 |
| 全名 | calbindin 2 |
| 背景介绍 | This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| 生物功能 | Calretinin is a calcium-binding protein which is abundant in auditory neurons. [UniProt] |
| 预测分子量 | 32 kDa |
检测图片 (5) Click the Picture to Zoom In
-
ARG10680 anti-Calretinin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10680 anti-Calretinin antibody (green) at 1:1000 dilution and costained with ARG10725 anti-Parvalbumin antibody [3C9] (red) at 1:1000 dilution. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
-
ARG10680 anti-Calretinin antibody IHC-Fr image
Immunohistochemistry: Frozen sections of adult Rat brain hippocampus (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained with ARG10680 anti-Calretinin antibody at 1:1000 (red), and co-stained with our rabbit anti-MeCP2 antibody (green). Calretinin labels a subset of hippocampal interneurons, which also express MeCP2 in the nucleus to give a yellow color.
-
ARG10680 anti-Calretinin antibody WB image
Western blot: 1) parvalbumin, 2) calretinin, 3) calbindin recombinant proteins, and 4) Rat brain lysates was stained with ARG10680 anti-Calretinin antibody at 1:1000 dilution.
-
ARG10680 anti-Calretinin antibody WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord and Cow spinal cord lysates stained with ARG10680 anti-Calretinin antibody (green) at 1:1000 dilution. The same blot was simultaneously stained with Mouse mAb to alpha-nternexin (red) at 1:10000 dilution.
-
ARG10680 anti-Calretinin antibody IHC-Fr image
Immunohistochemistry: Frozen sections of adult Rat cortex was stained with ARG10680 anti-Calretinin antibody (red) and co-stained with Mouse anti-calbindin antibody (green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Inset is a high-magnification image of the boxed area. Blue is DAPI staining that labels DNA.
