ARG67078

anti-Calreticulin antibody [SQab30350]

anti-Calreticulin antibody [SQab30350] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
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概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab30350] recognizes Calreticulin
反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab30350
同位型 IgG
靶点名称 Calreticulin
抗原物种 Human
抗原 Synthetic peptide of Calreticulin.
偶联标记 Un-conjugated
別名 CALR; Calreticulin; Calregulin; CC1qR; SSA; CRT; RO; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; FLJ26680; CALR1; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n; Autoantigen Ro; HEL-S-99n; CRTC

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100-1:200
WB1:500 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 55 kDa

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 811 Human CALR

Swiss-port # P27797 Human Calreticulin

基因名称 CALR
全名 Calreticulin
背景介绍 Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.
生物功能 Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER.
细胞定位 Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Lysosome, Sarcoplasmic reticulum, Secreted
产品亮点 Related products:
Calreticulin antibodies; Calreticulin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
预测分子量 48 kDa
翻译后修饰 Acetylation, Disulfide bond, Glycoprotein, Hydroxylation

检测图片 (2) Click the Picture to Zoom In

  • ARG67078 anti-Calreticulin antibody [SQab30350] IHC-P image

    Immunohistochemistry: Human prostate stained with ARG67078 anti-Calreticulin antibody [SQab30350].

  • ARG67078 anti-Calreticulin antibody [SQab30350] WB image (Customer review)

    Western blot: HeLa and HepG2 stained with ARG67078 anti-Calreticulin antibody [SQab30350] at 1:500 dilution.

客户反馈

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anti-Calreticulin antibody [SQab30350]

Application:WB

Sample:HeLa and HepG2

Sample Loading Amount:10 μL

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC