ARG57147
anti-Calpain S1 antibody [1D11]
anti-Calpain S1 antibody [1D11] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1D11] recognizes Calpain S1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D11 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Calpain S1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 84-268 of Human Calpain S1 |
| 偶联标记 | Un-conjugated |
| 別名 | CANPS; Calcium-dependent protease small subunit; CSS1; Calpain regulatory subunit; CALPAIN4; CDPS; CAPN4; Calcium-dependent protease small subunit 1; CANP small subunit; CANP; Calcium-activated neutral proteinase small subunit; Calpain small subunit 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAPNS1 |
| 全名 | calpain, small subunit 1 |
| 背景介绍 | This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| 生物功能 | Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57147 anti-Calpain S1 antibody [1D11] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57147 anti-Calpain S1 antibody [1D11] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57147 anti-Calpain S1 antibody [1D11] FACS image
Flow Cytometry: HeLa cell line stained with ARG57147 anti-Calpain S1 antibody [1D11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57147 anti-Calpain S1 antibody [1D11] WB image
Western blot: 50 ng of 1) CAPNS1 recombinant proteins, 40 µg of 2) HeLa, 3) A431, 4) 293T, 5) Balb/3T3, and 6) U87MG cell lysates stained with ARG57147 anti-Calpain S1 antibody [1D11] at 1:1000.
