ARG55065
anti-Calpain 6 antibody [1395CT446.22.5]
anti-Calpain 6 antibody [1395CT446.22.5] for Flow cytometry,Western blot and Mouse
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Calpain 6 |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1395CT446.22.5 |
| 同位型 | IgG1 |
| 靶点名称 | Calpain 6 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human Calpain 6. |
| 偶联标记 | Un-conjugated |
| 別名 | DJ914P14.1; CANPX; CalpM; Calpain-6; Calpain-like protease X-linked; Calpamodulin; CAPNX |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human placenta |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAPN6 |
| 全名 | calpain 6 |
| 背景介绍 | Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009] |
| 生物功能 | Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle. Note=During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 75 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG55065 anti-Calpain 6 antibody WB image
Western blot: 20 µg of Human placenta lysate stained with ARG55065 anti-Calpain 6 antibody at 1:1000 dilution.
-
ARG55065 anti-Calpain 6 antibody FACS image
Flow Cytometry: A549 cells stained with ARG55065 anti-Calpain 6 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
