ARG55063
anti-Calpain 2 antibody [1381CT669.7.66.71]
anti-Calpain 2 antibody [1381CT669.7.66.71] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Calpain 2 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1381CT669.7.66.71 |
| 同位型 | IgG2b |
| 靶点名称 | Calpain 2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide of Human Calpain 2. |
| 偶联标记 | Un-conjugated |
| 別名 | CANP2; Calpain-2 large subunit; Calpain-2 catalytic subunit; EC 3.4.22.53; Millimolar-calpain; M-calpain; mCANP; Calpain large polypeptide L2; Calpain M-type; CANP 2; CANPL2; CANPml; Calcium-activated neutral proteinase 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A549 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAPN2 |
| 全名 | calpain 2, (m/II) large subunit |
| 背景介绍 | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 80 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55063 anti-Calpain 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG55063 anti-Calpain 2 antibody at 1:25 dilution.
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ARG55063 anti-Calpain 2 antibody WB image
Western blot: 35 µg of A549 cell lysate stained with ARG55063 anti-Calpain 2 antibody at 1:1000 dilution.
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ARG55063 anti-Calpain 2 antibody FACS image
Flow Cytometry: U-87 MG cells stained with ARG55063 anti-Calpain 2 antibody (right histogram) at 1:100 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
