ARG45874
anti-Calcium binding protein P22 antibody
anti-Calcium binding protein P22 antibody for Western blot,ICC/IF,Flow cytometry and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Calcium binding protein P22 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Calcium binding protein P22 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to N-terminal region of human Calcium binding protein P22. |
| 偶联标记 | Un-conjugated |
| 別名 | Calcineurin Like EF-Hand Protein 1; CHP1; CHP; Calcineurin B homologous protein 1; Calcineurin B-like protein; Calcium-binding protein CHP; Calcium-binding protein p22; EF-hand calcium-binding domain-containing protein p22 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 34 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CHP1 |
| 全名 | Calcineurin Like EF-Hand Protein 1 |
| 背景介绍 | This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq, Jul 2008] |
| 生物功能 | Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na+/H+ exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na+/H+ exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner. [UniProt] |
| 细胞定位 | Cell membrane; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Membrane; Nucleus. [UniProt] |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Lipoprotein; Myristate; Phosphoprotein. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45874 anti-Calcium binding protein P22 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45874 anti-Calcium binding protein P22 antibody at 5 μg/ml dilution.
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ARG45874 anti-Calcium binding protein P22 antibody WB image
Western blot: RT-4 and SIHA stained with ARG45874 anti-Calcium binding protein P22 antibody at 0.5 μg/ml dilution.
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ARG45874 anti-Calcium binding protein P22 antibody FACS image
Flow Cytometry: K562 stained with ARG45874 anti-Calcium binding protein P22 antibody at 1 µg/10^6 cells dilution.
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ARG45874 anti-Calcium binding protein P22 antibody WB image
Western blot: Rat liver stained with ARG45874 anti-Calcium binding protein P22 antibody at 0.5 μg/ml dilution.
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ARG45874 anti-Calcium binding protein P22 antibody WB image
Western blot: Mouse liver stained with ARG45874 anti-Calcium binding protein P22 antibody at 0.5 μg/ml dilution.
