ARG64934
anti-CaMKII alpha antibody
anti-CaMKII alpha antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CaMKII alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CaMKII alpha |
| 抗原物种 | Human |
| 抗原 | C-PRTAQSEETRVWHR |
| 偶联标记 | Un-conjugated |
| 別名 | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30157 Phospho CaMKII Antibody Duo Related products: CaMKII alpha antibodies; CaMKII alpha Duos / Panels; Anti-Goat IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG64934 anti-CaMKII alpha antibody WB image
Western blot: Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG64934 anti-CaMKII alpha antibody at 0.1 µg/ml dilution.
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ARG64934 anti-CaMKII alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64934 anti-CaMKII alpha antibody WB image
Western blot: 35 µg of Human cerebellum (A), Mouse brain (B) and Rat brain (C) lysates (in RIPA buffer) stained with ARG64934 anti-CaMKII alpha antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64934 anti-CaMKII alpha antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Kelly cells permeabilized with 0.5% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64934 anti-CaMKII alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
