ARG22260

anti-CaMKII alpha antibody [6G9]

anti-CaMKII alpha antibody [6G9] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Mouse,Rat,Bovine

publication_link 参考文献1

概述

产品描述 Mouse Monoclonal antibody [6G9] recognizes CaMKII alpha
反应物种 Hu, Ms, Rat, Bov
应用 ELISA, ICC/IF, IHC, IP, RIA, WB
特异性 Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
宿主 Mouse
克隆 Monoclonal
克隆号 6G9
同位型 IgG1
靶点名称 CaMKII alpha
抗原物种 Rat
抗原 Partially purified Rat CaMKII
偶联标记 Un-conjugated
別名 CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
ICC/IF1:50
IHC1:2000
IPAssay-dependent
RIAAssay-dependent
WB1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂 0.09% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12322 Mouse CAMK2A

GeneID: 25400 Rat CAMK2A

GeneID: 815 Human CAMK2A

基因名称 Camk2a
全名 calcium/calmodulin-dependent protein kinase II alpha
背景介绍 The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
生物功能 CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). [UniProt]
细胞定位 Cytoplasm, Cell Junction, Mitochondrion, Nucleus, Presynaptic Cell Membrane, Synapse
预测分子量 54 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG22260 anti-CaMKII alpha antibody [6G9] ICC/IF image

    Immunocytochemistry: Mouse dissociated hippocampal neurons stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.

  • ARG22260 anti-CaMKII alpha antibody [6G9] WB image

    Western blot: Rat brain lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9].

  • ARG22260 anti-CaMKII alpha antibody [6G9] IHC image

    Immunohistochemistry: Formalin fixed colon carcinoma stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:10000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.

  • ARG22260 anti-CaMKII alpha antibody [6G9] WB image

    Western blot: Rat brain membrane lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.

文献引用

Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats

WB / Rat

Yu-Shun Lin et al.
J Nutr Biochem.,  (2021)

publication_link

 

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