ARG45821

anti-CYP51A1 / CYP51 antibody

anti-CYP51A1 / CYP51 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes CYP51A1 / CYP51
反应物种 Hu, Ms, Mk
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CYP51A1 / CYP51
抗原物种 Human
抗原 Recombinant protein containing to human CYP51A1 / CYP51.
偶联标记 Un-conjugated
別名 CYP51A1; Cytochrome P450 Family 51 Subfamily A Member 1; LDM; P450-14DM; P450L1; CYPL1; CYP51; CP51; Cytochrome P450, Family 51, Subfamily A, Polypeptide 1; Cytochrome P450, 51 (Lanosterol 14-Alpha-Demethylase); Lanosterol 14-Alpha Demethylase; Sterol 14-Alpha Demethylase; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; CYPLI; EC 1.14.14.154; EC 1.14.13.70 47

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 57 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1595 Human CYP51A1

Swiss-port # Q16850 Human Lanosterol 14-alpha demethylase

基因名称 CYP51A1
全名 Cytochrome P450 Family 51 Subfamily A Member 1
背景介绍 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
生物功能 Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. [UniProt]
细胞定位 Endoplasmic reticulum; Membrane; Microsome. [UniProt]
预测分子量 57 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG45821 anti-CYP51A1 / CYP51 antibody ICC/IF image

    Immunofluorescence: CACO-2 stained with ARG45821 anti-CYP51A1 / CYP51 antibody at 5 μg/ml dilution.

  • ARG45821 anti-CYP51A1 / CYP51 antibody WB image

    Western blot: A431 stained with ARG45821 anti-CYP51A1 / CYP51 antibody at 0.5 μg/ml dilution.

  • ARG45821 anti-CYP51A1 / CYP51 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45821 anti-CYP51A1 / CYP51 antibody at 1 µg/10^6 cells dilution.

  • ARG45821 anti-CYP51A1 / CYP51 antibody WB image

    Western blot: Mouse liver stained with ARG45821 anti-CYP51A1 / CYP51 antibody at 0.5 μg/ml dilution.

  • ARG45821 anti-CYP51A1 / CYP51 antibody WB image

    Western blot: COS-7 stained with ARG45821 anti-CYP51A1 / CYP51 antibody at 0.5 μg/ml dilution.