ARG22848
anti-CYP19A1 / Aromatase antibody [H4]
anti-CYP19A1 / Aromatase antibody [H4] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Baboon,Bovine,Chicken,Goat,Horse,Marmoset,Pig,Rabbit,Sheep
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody [H4] recognizes CYP19A1 / Aromatase Mouse anti Human Cytochrome P450 Aromatase antibody, clone H4 recognizes a conserved epitope within cytochrome P450 aromatase (P450 arom). P450 arom plays an important role in estrogen biosynthesis and is highly conserved amongst mammals.P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using Mouse anti Human Cytochrome P450 Aromatase antibody, clone H4 (Turner et al. 2002). |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bb, Bov, Chk, Goat, Hrs, Marmoset, Pig, Rb, Sheep |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | H4 |
| 同位型 | IgG2a |
| 靶点名称 | CYP19A1 / Aromatase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 376 - 390 of Human CYP19A1 / Aromatase. |
| 偶联标记 | Un-conjugated |
| 別名 | ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO |
应用说明
| 应用建议 |
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| 应用说明 | WB: This product detects a band of approximately 55 kDa in human placental extracts. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Tissue Culture Supernatant. |
| 缓冲液 | Tissue Culture Supernatant and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CYP19A1 |
| 全名 | cytochrome P450, family 19, subfamily A, polypeptide 1 |
| 背景介绍 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| 生物功能 | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. [UniProt] |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). |
检测图片 (1) Click the Picture to Zoom In
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ARG22848 anti-CYP19A1 / Aromatase antibody [H4] WB image
Western blot: Human placenta extract stained with ARG22848 anti-CYP19A1 / Aromatase antibody [H4].
文献引用
Remote Ischemia Postconditioning Mitigates Hippocampal Neuron Impairment by Modulating Cav1.2-CaMKIIα-Aromatase Signaling After Global Cerebral Ischemia in Ovariectomized Rats
WB, IHC-Fr / Rat
Coupling of GPR30 mediated neurogenesis and protection with astroglial Aromatase-STAT3 signaling in rat hippocampus after global cerebral ischemia
IHC-Fr / Mouse
