ARG64444
anti-CYP19A1 / Aromatase antibody
anti-CYP19A1 / Aromatase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CYP19A1 / Aromatase |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CYP19A1 / Aromatase |
| 抗原物种 | Human |
| 抗原 | C-HDLSLHPDETKN |
| 偶联标记 | Un-conjugated |
| 別名 | ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). |
检测图片 (2) Click the Picture to Zoom In
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ARG64444 anti-CYP19A1 / Aromatase antibody WB image
Western blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64444 anti-CYP19A1 / Aromatase antibody at 0.1 µg/ml dilution.
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ARG64444 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64444 anti-CYP19A1 / Aromatase antibody at 4 µg/ml dilution followed by AP-staining.
