ARG66853
anti-CYP11B1 + CYP11B2 antibody
anti-CYP11B1 + CYP11B2 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CYP11B1 + CYP11B2 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CYP11B1 + CYP11B2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from the C-terminal region of Human CYP11B1/2. |
| 偶联标记 | Un-conjugated |
| 別名 | CYP11B1: Cytochrome P-450c11; CYP11B; P450C11; FHI; Cytochrome P450C11; EC 1.14.15.4; Steroid 11-beta-hydroxylase; CYPXIB1; Cytochrome P450 11B1, mitochondrial; CPN1 CYP11B2: CYP11B; P-450C18; Cytochrome P-450Aldo; CYP11BL; Cytochrome P-450C18; ALDOS; Aldosterone-synthesizing enzyme; EC 1.14.15.4; EC 1.14.15.5; Cytochrome P450 11B2, mitochondrial; CYPXIB2; P450aldo; Steroid 18-hydroxylase; CPN2; P450C18; Aldosterone synthase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | COLO205 | ||||
| 实际分子量 | ~ 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CYP11B1; CYP11B2 |
|---|---|
| 全名 | cytochrome P450, family 11, subfamily B, polypeptide 1 cytochrome P450, family 11, subfamily B, polypeptide 2 |
| 背景介绍 | CYP11B gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP11B1 protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] CYP11B2 protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008] |
| 生物功能 | CYP11B1: A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids (PubMed:18215163). Catalyzes the hydroxylation of carbon hydrogen bond at 11-beta position of 11-deoxycortisol and 11-deoxycorticosterone/21-hydroxyprogesterone yielding cortisol or corticosterone, respectively (PubMed:18215163). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:18215163). [UniProt] CYP11B2: A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone (PubMed:11856349, PubMed:23322723, PubMed:1594605, PubMed:9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone (PubMed:11856349, PubMed:23322723, PubMed:1594605, PubMed:9814506). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:11856349, PubMed:23322723, PubMed:1594605, PubMed:9814506). [UniProt] |
| 细胞定位 | CYP11B1 & CYP11B2: Mitochondrion membrane. [UniProt] |
| 预测分子量 | 58 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG66853 anti-CYP11B1 + CYP11B2 antibody WB image
Western blot: COLO205 cell lysate stained with ARG66853 anti-CYP11B1 + CYP11B2 antibody.
