ARG65672
anti-CYB5R3 antibody
anti-CYB5R3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CYB5R3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 靶点名称 | CYB5R3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human CYB5R3 (CLPNLDHVGHPTER) |
| 偶联标记 | Un-conjugated |
| 別名 | NADH-cytochrome b5 reductase 3; Cytochrome b5 reductase; B5R; Diaphorase-1; EC 1.6.2.2; DIA1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CYB5R3 |
| 全名 | cytochrome b5 reductase 3 |
| 背景介绍 | This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010] |
| 生物功能 | Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 38.2 kDa (NP_001165131.1) |
检测图片 (2) Click the Picture to Zoom In
-
ARG65672 anti-CYB5R3 antibody WB image
Western blot: 35 µg of Human Umbilical Cord lysate stained with ARG65672 anti-CYB5R3 antibody at 0.01 µg/ml dilution (incubate 1 hour).
-
ARG65672 anti-CYB5R3 antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human Adrenal Gland stained with ARG65672 anti-CYB5R3 antibody at 3.8 µg/ml dilution. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
