ARG66104
anti-CXCL14 antibody
anti-CXCL14 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXCL14 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXCL14 |
| 抗原物种 | Human |
| 抗原 | E. coli derived recombinant Human CXCL14. (SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE) |
| 偶联标记 | Un-conjugated |
| 別名 | MIP-2G; KS1; BMAC; Small-inducible cytokine B14; KEC; C-X-C motif chemokine 14; MIP-2g; Chemokine BRAK; MIP2G; BRAK; NJAC; SCYB14 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCL14 |
| 全名 | chemokine (C-X-C motif) ligand 14 |
| 背景介绍 | This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014] |
| 生物功能 | Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. [UniProt] |
| 预测分子量 | 13 kDa |
| 翻译后修饰 | Ubiquitinated, followed by degradation by the proteasome. |
检测图片 (4) Click the Picture to Zoom In
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ARG66104 anti-CXCL14 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG66104 anti-CXCL14 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG66104 anti-CXCL14 antibody WB image
Western blot: 250 - 0.24 ng of Human BRAK stained with ARG66104 anti-CXCL14 antibody, under non-reducing conditions.
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ARG66104 anti-CXCL14 antibody standard curve image
Sandwich ELISA: ARG66104 anti-CXCL14 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66105 anti-CXCL14 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66104 anti-CXCL14 antibody WB image
Western blot: 250 - 0.24 ng of Human BRAK stained with ARG66104 anti-CXCL14 antibody, under reducing conditions.
