ARG58540
anti-CXADR / CAR antibody
anti-CXADR / CAR antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXADR / CAR |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXADR / CAR |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 132-161 (Center) of Human CXADR / CAR. |
| 偶联标记 | Un-conjugated |
| 別名 | Coxsackievirus B-adenovirus receptor; CAR4/6; HCAR; hCAR; CAR; Coxsackievirus and adenovirus receptor; CVB3-binding protein; HCVADR |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | NCI-H460 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P78310 Human Coxsackievirus and adenovirus receptor |
|---|---|
| 基因名称 | CXADR |
| 全名 | coxsackie virus and adenovirus receptor |
| 背景介绍 | The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011] |
| 生物功能 | Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Basolateral cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface Isoform 3: Secreted. Isoform 5: Secreted. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | N-glycosylated. Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58540 anti-CXADR / CAR antibody WB image
Western blot: 35 µg of NCI-H460 cell lysate stained with ARG58540 anti-CXADR / CAR antibody.
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ARG58540 anti-CXADR / CAR antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG58540 anti-CXADR / CAR antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
