ARG58468

anti-CTH / Cystathionase antibody

anti-CTH / Cystathionase antibody for Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes CTH / Cystathionase
反应物种 Hu, Ms
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CTH / Cystathionase
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-405 of Human CTH / Cystathionase (NP_001893.2).
偶联标记 Un-conjugated
別名 EC 4.4.1.1; Cysteine-protein sulfhydrase; Gamma-cystathionase; Cystathionine gamma-lyase

应用说明

应用建议
应用 推荐稀释比
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HepG2
实际分子量 45 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 107869 Mouse CTH

GeneID: 1491 Human CTH

Swiss-port # P32929 Human Cystathionine gamma-lyase

Swiss-port # Q8VCN5 Mouse Cystathionine gamma-lyase

基因名称 CTH
全名 cystathionine gamma-lyase
背景介绍 This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
生物功能 Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 45 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG58468 anti-CTH / Cystathionase antibody WB image

    Western blot: 25 µg of HepG2 cell lysate stained with ARG58468 anti-CTH / Cystathionase antibody at 1:1000 dilution.