ARG64952

anti-CSX1 / NKX2-5 antibody

anti-CSX1 / NKX2-5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody

概述

产品描述 Goat Polyclonal antibody recognizes CSX1 / NKX2-5
反应物种 Hu, Ms
预测物种 Cow, Rat, Dog
应用 IHC-P, WB
特异性 This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 CSX1 / NKX2-5
抗原物种 Human
抗原 C-PRAYSDPDPAKDPR
偶联标记 Un-conjugated
別名 NKX2.5; NKX2E; Homeobox protein Nkx-2.5; CHNG5; NKX4-1; VSD3; CSX; CSX1; Homeobox protein NK-2 homolog E; HLHS2; Homeobox protein CSX; Cardiac-specific homeobox

应用说明

应用建议
应用 推荐稀释比
IHC-PAssay - dependent
WB0.1 - 0.5 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1482 Human NKX2-5

GeneID: 18091 Mouse NKX2-5

Swiss-port # P42582 Mouse Homeobox protein Nkx-2.5

Swiss-port # P52952 Human Homeobox protein Nkx-2.5

背景介绍 This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
研究领域 Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody
预测分子量 35 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG64952 anti-CSX1 / NKX2-5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64952 anti-CSX1 / NKX2-5 antibody at 6 µg/ml dilution followed by AP-staining.

  • ARG64952 anti-CSX1 / NKX2-5 antibody WB image

    Western blot: 35 µg of Mouse heart lysate (in RIPA buffer) stained with ARG64952 anti-CSX1 / NKX2-5 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.