ARG42070
anti-CROT / COT antibody
anti-CROT / COT antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CROT / COT |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CROT / COT |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 49-79 of Human CROT. |
| 偶联标记 | Un-conjugated |
| 別名 | Peroxisomal carnitine O-octanoyltransferase; COT; EC 2.3.1.137 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse liver | ||||||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9DC50 Mouse Peroxisomal carnitine O-octanoyltransferase Swiss-port # Q9UKG9 Human Peroxisomal carnitine O-octanoyltransferase |
|---|---|
| 基因名称 | CROT |
| 全名 | carnitine O-octanoyltransferase |
| 背景介绍 | This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
| 生物功能 | Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. [UniProt] |
| 细胞定位 | Peroxisome. [UniProt] |
| 预测分子量 | 70 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42070 anti-CROT / COT antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG42070 anti-CROT / COT antibody.
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ARG42070 anti-CROT / COT antibody WB image
Western blot: 35 µg of Mouse liver lysate stained with ARG42070 anti-CROT / COT antibody.
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ARG42070 anti-CROT / COT antibody FACS image
Flow Cytometry: K562 cells stained with ARG42070 anti-CROT / COT antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
