ARG65172
anti-CRISP2 antibody
anti-CRISP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CRISP2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CRISP2 |
| 抗原物种 | Human |
| 抗原 | C-QHSDPEDRKTSTR |
| 偶联标记 | Un-conjugated |
| 別名 | TPX1; TSP1; Testis-specific protein TPX-1; Cancer/testis antigen 36; Cysteine-rich secretory protein 2; GAPDL5; CT36; CRISP-2 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CRISP2 |
| 全名 | cysteine-rich secretory protein 2 |
| 生物功能 | May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation. [UniProt] |
| 研究领域 | Developmental Biology antibody |
| 预测分子量 | 27 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG65172 anti-CRISP2 antibody WB image
Western blot: 10 µg of HEK293 cell lysate (in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag stained with ARG65172 anti-CRISP2 antibody at 1 µg/ml dilution (Lane A); Mock-transfected HEK293 stained with primary antibodies (Lane B); Overexpressing HEK293 cell lysate stained with anti-MYC Tag at 1:1000 dilution (Lane C). Primary antibodies were incubated at RT for 1 hour.
-
ARG65172 anti-CRISP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65172 anti-CRISP2 antibody at 3.75 µg/ml dilution followed by AP-staining.
