ARG42600

anti-CREB3L2 / BBF2H7 antibody

anti-CREB3L2 / BBF2H7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CREB3L2 / BBF2H7
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog, Gpig, Hrs, Rb
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CREB3L2 / BBF2H7
抗原物种 Human
抗原 Synthetic peptide around the N-terminal region of Human CREB3L2 / BBF2H7. (within the following region: HSYSLCEEPRAQSPFTHITSDSFNDDEVESEKWYLSTDF)
偶联标记 Un-conjugated
別名 Cyclic AMP-responsive element-binding protein 3-like protein 2; BBF2 human homolog on chromosome 7; BBF2H7; cAMP-responsive element-binding protein 3-like protein 2

应用说明

预测反应性说明 Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 85%; Guinea pig: 87%; Horse: 87%; Mouse: 86%; Rabbit: 87%; Rat: 87%
应用建议
应用 推荐稀释比
IHC-PAssay-dependent
WB1 - 5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Jurkat, Human fetal muscle, lung, heart and brain.
实际分子量 ~ 52 kDa

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.09% (w/v) Sodium azide and 2% Sucrose.
抗菌剂 0.09% (w/v) Sodium azide
稳定剂 2% Sucrose
浓度 Batch dependent: 0.5 - 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 64764 Human CREB3L2

Swiss-port # Q70SY1 Human Cyclic AMP-responsive element-binding protein 3-like protein 2

基因名称 CREB3L2
全名 cAMP responsive element binding protein 3-like 2
背景介绍 This gene encodes a member of the oasis bZIP transcription factor family. Members of this family can dimerize but form homodimers only. The encoded protein is a transcriptional activator. Translocations between this gene on chromosome 7 and the gene fused in sarcoma on chromosome 16 can be found in some tumors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
生物功能 Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes (By similarity). In a neuroblastoma cell line, protects cells from ER stress-induced death (PubMed:17178827). In vitro activates transcription of target genes via direct binding to the CRE site (PubMed:17178827). [UniProt]
细胞定位 Endoplasmic reticulum membrane; Single-pass type II membrane protein. Note=ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus. Processed cyclic AMP-responsive element-binding protein 3-like protein 2: Nucleus. Note=Upon ER stress, translocated into the nucleus. [UniProt]
预测分子量 57 kDa
翻译后修饰 Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).

N-glycosylated.

Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody.

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody at 1.25 µg/ml dilution.

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody WB image

    Western blot: Human fetal muscle lysate stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody at 1 µg/ml dilution.

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody WB image

    Western blot: Human fetal lung lysate stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody at 1 µg/ml dilution.

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody WB image

    Western blot: Human fetal heart lysate stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody at 1 µg/ml dilution.

  • ARG42600 anti-CREB3L2 / BBF2H7 antibody WB image

    Western blot: Human fetal brain lysate stained with ARG42600 anti-CREB3L2 / BBF2H7 antibody at 1 µg/ml dilution.