ARG59917
anti-CRBN antibody
anti-CRBN antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CRBN |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CRBN |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-280 of Human CRBN (NP_001166953.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Protein cereblon; MRT2A; MRT2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Rat brain, Mouse spleen and A549 | ||||
| 实际分子量 | 51 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CRBN |
| 全名 | cereblon |
| 背景介绍 | This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| 生物功能 | Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59917 anti-CRBN antibody WB image
Western blot: 25 µg of Rat brain, Mouse spleen and A549 cell lysates stained with ARG59917 anti-CRBN antibody at 1:1000 dilution.
