ARG56564
anti-CRB3 antibody [14F9]
anti-CRB3 antibody [14F9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rat Monoclonal antibody [14F9] recognizes CRB3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 14F9 |
| 同位型 | IgG2c |
| 靶点名称 | CRB3 |
| 抗原物种 | Human |
| 抗原 | Full length of Human CRB3 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein crumbs homolog 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified. |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CRB3 |
| 全名 | crumbs family member 3 |
| 背景介绍 | This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. [UniProt] |
| 预测分子量 | 13 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG56564 anti-CRB3 antibody [14F9] ICC/IF image
Immunofluorescence: Overexpressed human CRB3 in lysates of HeLa cells stained with ARG56564 anti-CRB3 antibody [14F9].
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ARG56564 anti-CRB3 antibody [14F9] WB image
Western blot: analysis of full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra). ARG56564 anti-CRB3 antibody [14F9] was used at 1:1000 dilution.
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ARG56564 anti-CRB3 antibody [14F9] ICC/IF image
Immunofluorescence: Endogenous human CRB3 expression in the mouse intestine stained with ARG56564 anti-CRB3 antibody [14F9]. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.
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ARG56564 anti-CRB3 antibody [14F9] WB image
Western blot: analysis of overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). The blots were stained with ARG56564 anti-CRB3 antibody [14F9].
