ARG56564

anti-CRB3 antibody [14F9]

anti-CRB3 antibody [14F9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Rat Monoclonal antibody [14F9] recognizes CRB3
反应物种 Hu, Ms
应用 ICC/IF, IHC-P, WB
宿主 Rat
克隆 Monoclonal
克隆号 14F9
同位型 IgG2c
靶点名称 CRB3
抗原物种 Human
抗原 Full length of Human CRB3 protein.
偶联标记 Un-conjugated
別名 Protein crumbs homolog 3

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100
IHC-P1:100
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified.
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 224912 Mouse CRB3

GeneID: 92359 Human CRB3

Swiss-port # Q8QZT4 Mouse Protein crumbs homolog 3

Swiss-port # Q9BUF7 Human Protein crumbs homolog 3

基因名称 CRB3
全名 crumbs family member 3
背景介绍 This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
生物功能 Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. [UniProt]
预测分子量 13 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG56564 anti-CRB3 antibody [14F9] ICC/IF image

    Immunofluorescence: Overexpressed human CRB3 in lysates of HeLa cells stained with ARG56564 anti-CRB3 antibody [14F9].

  • ARG56564 anti-CRB3 antibody [14F9] WB image

    Western blot: analysis of full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra). ARG56564 anti-CRB3 antibody [14F9] was used at 1:1000 dilution.

  • ARG56564 anti-CRB3 antibody [14F9] ICC/IF image

    Immunofluorescence: Endogenous human CRB3 expression in the mouse intestine stained with ARG56564 anti-CRB3 antibody [14F9]. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.

  • ARG56564 anti-CRB3 antibody [14F9] WB image

    Western blot: analysis of overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). The blots were stained with ARG56564 anti-CRB3 antibody [14F9].