ARG56184
anti-CPS1 antibody [CPS1/1022]
anti-CPS1 antibody [CPS1/1022] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody [CPS1/1022] recognizes CPS1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CPS1/1022 |
| 同位型 | IgG1 |
| 靶点名称 | CPS1 |
| 抗原物种 | Human |
| 抗原 | Recombinant human CPS1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | CPSASE1; Carbamoyl-phosphate synthase [ammonia], mitochondrial; CPSase I; PHN; Carbamoyl-phosphate synthetase I; EC 6.3.4.16 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P31327 Human Carbamoyl-phosphate synthase [ammonia], mitochondrial |
|---|---|
| 基因名称 | CPS1 |
| 全名 | carbamoyl-phosphate synthase 1, mitochondrial |
| 背景介绍 | The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.[provided by RefSeq, May 2010] |
| 生物功能 | Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. [UniProt] |
| 细胞定位 | Finely granular cytoplasmic |
| 预测分子量 | 165 kDa |
| 翻译后修饰 | Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus. Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity). Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation. |
检测图片 (1) Click the Picture to Zoom In
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ARG56184 anti-CPS1 antibody [CPS1/1022] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocellular carcinoma stained with ARG56184 anti-CPS1 antibody [CPS1/1022].
